CD8 alpha Antikörper (FITC)

Produktnummer ABIN6655402

Hersteller Produkt- Nr.: 200-302-n61

Der Maus Monoklonal anti-CD8 alpha Antikörper wird verwendet zum Nachweis von CD8 alpha in Proben von Human. Er wurde validiert für FACS und IHC.

Kurzübersicht für CD8 alpha Antikörper (FITC) (ABIN6655402)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: HIT8a

Produktdetails anti-CD8 alpha Antikörper (FITC)

Hersteller Produkt- Nr.: 200-302-n61

Hersteller: Rockland

Verwendungszweck: CD8a Fluorescein Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD8a, Marmoset, and chimpanzee.

Aufreinigung: Fluorescein conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a.

Sterilität: Sterile filtered

Immunogen: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.

Isotyp: IgG1 kappa

Labeling Ratio: 4-6

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Kommentare:

Anti-CD8a has been tested in Flow Cytometry and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8a

Hintergrund:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a

Background: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Gene Name: CD8A

Gen-ID: 925

NCBI Accession: NP_001139345

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg