DDIT4 Antikörper (pThr23, pThr25)

Produktnummer ABIN6655385

Hersteller Produkt- Nr.: 600-401-x24

Dieses Anti-DDIT4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DDIT4 in WB und ELISA. Geeignet für Human.

Kurzübersicht für DDIT4 Antikörper (pThr23, pThr25) (ABIN6655385)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDIT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-DDIT4 Antikörper

Bindungsspezifität: pThr23, pThr25

Hersteller Produkt- Nr.: 600-401-x24

Hersteller: Rockland

Verwendungszweck: REDD1 Phospho T23/25 Antibody

Kreuzreaktivität (Details): Anti-REDD1 pT23/pT25 Antibody is directed against REDD1 protein phosphorylated at T23 and T25.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Thr23/25 REDD1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:5,000

Western_Blot_Dilution: 1:500

Kommentare:

Anti-Phospho-Thr23/25 REDD1 Antibody is tested for Western Blots and is specific for the ~34 kDa REDD1 phosphorylated at Thr23/25. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Details zu DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Andere Bezeichnung: REDD1

Hintergrund:

Synonyms: DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1

Background: REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli. REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress. It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A-DDB1-ROC1-b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b. REDD1 pT23/25 Antibody is ideal for researchers interested in cancer research.

Gene Name: DDIT4

Gen-ID: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process