CD20 Antikörper (APC)

Produktnummer ABIN6655312

Produktdetails anti-CD20 Antikörper (APC)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD20 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity with CD20 from other sources has not been tested

Aufreinigung: Allophycocyanin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20. Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey. . Anti-CD20 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Immunogen:

Immunogen: Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.

Klon: 2H7

Isotyp: IgG2b

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD20 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Referenzen für anti-CD20 Antikörper (APC) (ABIN6655312)

Tedder, Engel: "CD20: a regulator of cell-cycle progression of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 450-4, (1994) (PubMed).

Hultin, Hausner, Hultin, Giorgi: "CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes." in: Cytometry, Vol. 14, Issue 2, pp. 196-204, (1993) (PubMed).

Details zu CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Andere Bezeichnung: CD20 (MS4A1 Produkte)

Synonyme: B1 antikoerper, Bp35 antikoerper, CD20 antikoerper, CVID5 antikoerper, LEU-16 antikoerper, MS4A2 antikoerper, S7 antikoerper, AA960661 antikoerper, Cd20 antikoerper, Ly-44 antikoerper, Ms4a2 antikoerper, MS4A1 antikoerper, bp35 antikoerper, cd20 antikoerper, ms4a2 antikoerper, leu-16 antikoerper, ms4a4c antikoerper, cd20-like antikoerper, membrane spanning 4-domains A1 antikoerper, membrane-spanning 4-domains, subfamily A, member 1 antikoerper, B-lymphocyte antigen CD20 antikoerper, MS4A1 antikoerper, Ms4a1 antikoerper, ms4a1 antikoerper, LOC101694281 antikoerper

Hintergrund:

Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20

Background: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 Molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Gene Name: MS4A1

Gen-ID: 931

NCBI Accession: NP_068769

UniProt: P11836