SMAD6 Antikörper

Produktnummer ABIN6655220

Hersteller Produkt- Nr.: 200-401-i15

Dieses Anti-SMAD6-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SMAD6 in WB, IHC und IF. Geeignet für Human.

Kurzübersicht für SMAD6 Antikörper (ABIN6655220)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SMAD6 Antikörper

Hersteller Produkt- Nr.: 200-401-i15

Hersteller: Rockland

Verwendungszweck: SMAD6 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-SMAD6 from Human, Mouse, New World monkey, Rat and Sheep based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-SMAD6 Antibody was purified by Protein G chromatography.

Immunogen: SMAD6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to two internal amino acid sequences in the human SMAD6 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 1-3 μg/mL

Kommentare:

Anti-SMAD6 antibody is tested for use in IHC, IF, and WB. Expect a band approximately 53kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Andere Bezeichnung: SMAD6

Hintergrund:

Synonyms: MADH6

Background: Anti-SMAD6 antibody detects human SMAD6. SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Anti-SMAD6 antibody is ideal for investigators involved kinase and phosphatase and NFkappaB research.

Gene Name: SMAD6

Gen-ID: 4091

NCBI Accession: NP_001136333

UniProt: O43541

Pathways: Chromatin Binding