MBD1 Antikörper (N-Term)

Produktnummer ABIN6655183

Hersteller Produkt- Nr.: 600-401-x42

Der Kaninchen Polyklonal Anti-MBD1-Antikörper wurde für WB, ELISA und ChIP validiert. Er ist geeignet, MBD1 in Proben von Human zu detektieren.

Kurzübersicht für MBD1 Antikörper (N-Term) (ABIN6655183)

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-MBD1 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 600-401-x42

Hersteller: Rockland

Verwendungszweck: MBD1 Antibody

Kreuzreaktivität (Details): Cross reactivity with other species was not tested.

Aufreinigung: Anti-MBD1 Antibody was purified by affinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-MBD1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminus of human MBD1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ChIP_Dilution: 1.5 μg per IP

ELISA_Dilution: 1:1,000

Western_Blot_Dilution: 1:500

Kommentare:

Anti-MBD1 Antibody is tested for Chromatin Immunoprecipitation, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kDa in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Konservierungsmittel: ProClin, Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Andere Bezeichnung: MBD1

Hintergrund:

Synonyms: Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, CXXC3, PCM1

Background: MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC's) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription. Anti-MBD1 Antibody is ideal for research in Epigenetics, Gene Expression and Chromatin Remodeling.

Gene Name: MBD1

Gen-ID: 4152

NCBI Accession: NP_001191065

UniProt: Q9UIS9