CD28 Antikörper (PE)

Produktnummer ABIN6655121

Hersteller Produkt- Nr.: 200-308-n66

Produktdetails anti-CD28 Antikörper (PE)

Target: CD28

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD28 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-308-n66

Hersteller: Rockland

Verwendungszweck: CD28 Phycoerythrin Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD28, Baboon, Chimpanzee, Cynomolgus, and Rhesus.

Aufreinigung: Phycoerythrin conjugated CD28 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD28.

Sterilität: Sterile filtered

Immunogen: Anti-CD28 Antibody (Monoclonal) was produced by repeated immunizations with CD28 antigen.

Klon: CD28-2

Isotyp: IgG1 kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

Kommentare:

Anti-CD28 is tested for Flow Cytometry and is useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28 (CD28 Produkte)

Hintergrund:

Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD28

Background: The CD28 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA. The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo.

Gene Name: CD28

Gen-ID: 940

NCBI Accession: NP_001230006

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response