Integrin beta 3 Antikörper (C-Term)

Produktnummer ABIN6655109

Hersteller Produkt- Nr.: 600-401-gu0

Dieses Anti-Integrin beta 3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Integrin beta 3 in WB, IHC, FACS, ELISA, IP und FM. Geeignet für Human.

Kurzübersicht für Integrin beta 3 Antikörper (C-Term) (ABIN6655109)

Target: Integrin beta 3 (ITGB3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Integrin beta 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Produktdetails anti-Integrin beta 3 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-gu0

Hersteller: Rockland

Verwendungszweck: Integrin beta 3 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Integrin beta 3.

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Integrin beta 3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human Integrin beta 3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 5.0 μg/mL

Immunohistochemistry_Dilution: 1:100 - 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: Not recommended

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Integrin beta-3 Antibody has been tested in Western Blot, ELISA and IHC. Positive control lysates used U-87 p/n (W09-001-GX2) and MDA-MB-435 p/n (W09-001-A39) @ 1μg/mL in WB. Expect possible bands at ~80, 100 and 130 kDa in western blot using appropriate lysates. Immunohistochemistry application with human breast carcinoma tissue is recommended. Although not tested, this antibody is likely functional in flow cytometry and immunoprecipitation. This antibody is not recommended in immunofluorescence detection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Andere Bezeichnung: Integrin beta 3

Hintergrund:

Synonyms: rabbit anti-Integrin beta 3 antibody, Integrin Subunit beta 3, IGTB3, GP3A, Platelet membrane glycoprotein IIIa, GPIIIa, Integrin beta-3, CD61, CD61 Antibody

Background: Integrin beta 3, also called ITB3, belongs to the integrin beta chain family. Integrin alpha-V/beta-3 (ITGAV/ITB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B/ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. ITGA2B/ITGB3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. ITGAV/ITB3 binds to CX3CL1, NRG1, FGF1, IGF1, PLA2G2A, and FBN1. ITGAV/ITGB3 acts as a receptor for herpes virus 8/HHV-8, coxsackievirus A9, Hantaan virus, cytomegalovirus/HHV-5, human metapneumovirus, human parechovirus 1, and west nile virus. Intergrin beta 3 may be associated with disorders such as Glanzmann thrombasthenia, myocardial infarction, purpura, and insulin-like growth factor i deficency. Anti-Integrin beta 3 Antibody is useful for researched interested in RET Signaling, MAPK-Erk Pathway, ERK Signaling, and Integrin Pathway Research.

Gene Name: ITGB3

Gen-ID: 3690

NCBI Accession: NP_000203

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex