Cyclin L1 Antikörper (AA 314-369)

Produktnummer ABIN6655105

Produktdetails anti-Cyclin L1 Antikörper

Target: Cyclin L1 (CCNL1)

Bindungsspezifität: AA 314-369

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96 % homology with the protein from these sourcesCross reactivity with Cyclin L1a homologues from other sources has not been determined.

Aufreinigung: The product was purified from monospecific antiserum by Protein A chromatography. This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1a homologues from other sources has not been determined.

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 10 mg/mL - 20 μg/mL

Application Note: This Protein A purified antibody has been tested for use in ELISA, immunohistochemistry, western blot, and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 59 kDa in size corresponding to Cyclin L1a by western blotting in the appropriate cell lysate or extract.

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:2,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Referenzen für anti-Cyclin L1 Antikörper (ABIN6655105)

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Details zu Cyclin L1

Target: Cyclin L1 (CCNL1)

Andere Bezeichnung: Cyclin L1 (CCNL1 Produkte)

Synonyme: ANIA6A antikoerper, PRO1073 antikoerper, ania-6a antikoerper, 2610030E23Rik antikoerper, AU018493 antikoerper, Ccnl antikoerper, Ania-6a antikoerper, ccnl1 antikoerper, fd99b09 antikoerper, wu:fd99b09 antikoerper, zgc:55544 antikoerper, MCM2 antikoerper, cyclin-L1 antikoerper, MGC132032 antikoerper, bm-001 antikoerper, pro1073 antikoerper, MGC108436 antikoerper, Ccnl1 antikoerper, cyclin L1 antikoerper, cyclin L1b antikoerper, cyclin-L1 antikoerper, cyclin L1 L homeolog antikoerper, cyclin L1 S homeolog antikoerper, CCNL1 antikoerper, Ccnl1 antikoerper, ccnl1b antikoerper, LOC485730 antikoerper, ccnl1.L antikoerper, ccnl1 antikoerper, LOC100136245 antikoerper, ccnl1.S antikoerper

Hintergrund:

Synonyms: rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL1

Gen-ID: 57018

NCBI Accession: NP_064703

UniProt: Q9UK58