NEFH Antikörper

Produktnummer ABIN6655041

Hersteller Produkt- Nr.: 200-901-d82

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch NEFH in WB, IF und FM. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für NEFH Antikörper (ABIN6655041)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Rind (Kuh)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-NEFH Antikörper

Hersteller Produkt- Nr.: 200-901-d82

Hersteller: Rockland

Verwendungszweck: Neurofilament H Antibody

Kreuzreaktivität (Details): Anti-NF-H antibody is directed against NF-H.

Aufreinigung: The antibody is a total IgY fraction.

Immunogen: Anti-Neurofilament NF-H Antibody was produced by repeated immunizations with purified bovine NF-H.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1:25000

Western_Blot_Dilution: 1:50000

Other: User Optimized

Kommentare:

Anti-Neurofilament H antibody is tested for use in Western blotting and IF. Expect a band of approximately 200 kDa in size corresponding to the NF-H proteins in Western blots in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament H

Hintergrund:

Synonyms: Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

Background: NF-H antibody detects Neurofilament H. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-Neurofilament H antibody is ideal for investigators involved in Cell Signaling, and Neuroscience research.

Gene Name: NEFH

Gen-ID: 4744

UniProt: P12036