pan Keratin Antikörper
Kurzübersicht für pan Keratin Antikörper (ABIN6654984)
Target
Alle pan Keratin (panKRT) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Aufreinigung
- Purified
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Reinheit
- Protein A affinity chromatography
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Immunogen
- A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.
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Isotyp
- IgG kappa
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Applikationshinweise
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Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.
1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Konservierungsmittel
- Azide free
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- pan Keratin (panKRT)
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Andere Bezeichnung
- Multi Cytokeratin
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Hintergrund
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Target
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