Rekombinanter MUC1 Antikörper

Produktnummer ABIN6654784

Der Maus Monoklonal anti-MUC1 Antikörper wird verwendet zum Nachweis von MUC1 in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Rekombinanter MUC1 Antikörper (ABIN6654784)

Target: MUC1 (Mucin 1 (MUC1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: RMUC1-960

Produktdetails anti-MUC1 Antikörper

Verwendungszweck: Recombinant MUC-1 Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: Human milk fat globule membranes were used as the immunogen for this recombinant MUC-1 antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant MUC-1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant MUC-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC-1

Hintergrund: This mAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling