POLR2A/RPB1 Antikörper (pSer5)

Produktnummer ABIN6654646

Der Maus Monoklonal Anti-POLR2A/RPB1-Antikörper wurde für WB, IF und IHC (p) validiert. Er ist geeignet, POLR2A/RPB1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für POLR2A/RPB1 Antikörper (pSer5) (ABIN6654646)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CTD4H8

Produktdetails anti-POLR2A/RPB1 Antikörper

Bindungsspezifität: pSer5

Aufreinigung: Purified

Reinheit: Protein G affinity chromatography

Immunogen: The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant POLR2A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the POLR2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: POLR2A / RNA polymerase II subunit B1

Hintergrund: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Pathways: Regulatorische RNA Pathways