ANO1 Antikörper (AA 2-101)

Produktnummer ABIN6654568

Der Kaninchen Monoklonal Anti-ANO1-Antikörper wurde für IHC (p) validiert. Er ist geeignet, ANO1 in Proben von Human zu detektieren.

Kurzübersicht für ANO1 Antikörper (AA 2-101) (ABIN6654568)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ANO1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: DG1-2564R

Produktdetails anti-ANO1 Antikörper

Bindungsspezifität: AA 2-101

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: A portion of amino acids 2-101 from the human protein was used as the immunogen for this recombinant DOG1 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant DOG1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant DOG1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Andere Bezeichnung: DOG1 / TMEM16A / ANO1

Hintergrund: Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST's is nearly identical: 94.4 % vs. 94.7 %.