NEFH Antikörper

Produktnummer ABIN6654554

Dieses Anti-NEFH-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von NEFH in WB und IHC (p). Geeignet für Human, Ratte, Maus, Huhn und Schwein.

Kurzübersicht für NEFH Antikörper (ABIN6654554)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Schwein

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: NEFL-H-2324R

Produktdetails anti-NEFH Antikörper

Aufreinigung: Purified

Reinheit: Protein A affinity

Immunogen: Recombinant human protein (heavy subunit) was used as the immunogen for the recombinant Neurofilament antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the recombinant Neurofilament antibody to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament (-Heavy) / NF-H / NEFH

Hintergrund: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.