Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) Antikörper

Produktnummer ABIN6654518

Dieses Anti--Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von in WB, FACS und IHC (p). Geeignet für Human.

Kurzübersicht für Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) Antikörper (ABIN6654518)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPTBN2-2894R

Produktdetails

Bindungsspezifität: AA 356-475

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Flow cytometry: 1-2 μg/10^6 cells in 0.1ml,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Spectrin beta III antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Andere Bezeichnung: SPTBN2 / Spectrin beta III

Hintergrund: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis