SFPQ Antikörper (C-Term)

Produktnummer ABIN657824

Der Kaninchen Polyklonal Anti-SFPQ-Antikörper wurde für WB validiert. Er ist geeignet, SFPQ in Proben von Human zu detektieren.

Kurzübersicht für SFPQ Antikörper (C-Term) (ABIN657824)

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SFPQ Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB34049

Produktdetails anti-SFPQ Antikörper

Bindungsspezifität: AA 676-705, C-Term

Homologie: M

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SFPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 676-705 amino acids from the C-terminal region of human SFPQ.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: SFPQ Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Haltbarkeit: 6 months

Details zu SFPQ

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Andere Bezeichnung: SFPQ

Hintergrund: DNA-and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing, in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1, in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends, in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

Molekulargewicht: 76149

Gen-ID: 6421

NCBI Accession: NP_005057

UniProt: P23246