ESR2 Antikörper (AA 1-153)

Produktnummer ABIN189996

Der Maus Monoklonal anti-ESR2 Antikörper (Klon 6A12) (ABIN189996) detektiert spezifisch ESR2 in WB, IHC, IF/ICC und ChIP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ESR2 Antikörper (AA 1-153) (ABIN189996)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC), Chromatin Immunoprecipitation (ChIP)

Klon: 6A12

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: AA 1-153

Verwendungszweck: Anti-Estrogen Receptor beta Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human ER-b, a novel receptor (approx. 52 kD) recently isolated from testis. The size and structure of ER-b are very similar to ER-alpha with the ligand and DNA binding domains being highly conserved. The N-terminus of each receptor, which serves as their transactivation domain, are significantly different. Like ER-alpha, ER-b binds to estrogen with a high affinity and regulates estrogen-specific gene activation through direct interaction with estrogen response elements.

Kreuzreaktivität: Human

Immunogen: 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1-5 μg/mL.
Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4

Lagerung: -80 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: Estrogen receptor beta

Hintergrund: Estrogen receptor beta,Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {PubMed:20074560, PubMed:29261182, PubMed:30113650, PubMed:9325313}., [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {PubMed:9671811}.,Nucleus,ER-beta, Nuclear receptor subfamily 3 group A member 2

Gen-ID: 2100

NCBI Accession: NP_001035365

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling