RabbitBP4 / RabbitAP48 (AA 1-425) Antikörper

Produktnummer ABIN189974

Der Maus Monoklonal anti-RabbitBP4 / RabbitAP48 Antikörper wird verwendet zum Nachweis von RabbitBP4 / RabbitAP48 in Proben von Human. Er wurde validiert für IF/ICC, IHC (p), IP, WB, BP, ChIP, Func und IHC.

Kurzübersicht für RabbitBP4 / RabbitAP48 (AA 1-425) Antikörper (ABIN189974)

Target: RabbitBP4 / RabbitAP48

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Blocking Peptide (BP), Chromatin Immunoprecipitation (ChIP), Functional Studies (Func), Immunohistochemistry (IHC)

Klon: 11G10

Produktdetails

Bindungsspezifität: AA 1-425

Verwendungszweck: Anti-RbAp48 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human and mouse RbAp48.

Kreuzreaktivität: Human

Immunogen: GST fusion protein corresponding to aa 1-425 of human RbAp48.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable at -20°C for at least one (1) year, store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Antigendetails

Target: RabbitBP4 / RabbitAP48

Hintergrund: Retinoblastoma-associated protein,Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}., (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.,Nucleus,p105-Rb, p110-RB1, pRb, Rb, pp110

Gen-ID: 5925

NCBI Accession: NP_000312

UniProt: P06400