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MITF Antikörper (N-Term)

Name: MITF Antikörper (N-Term)
SKU: ABIN655506
Price: 434.77 EUR
Availability: InStock

MITF Reaktivität: Human WB, IF, FACS Wirt: Kaninchen Polyclonal RB18810 unconjugated

Anti-MITF Antibody (N-term)at 1:2000 dilution + whole cell lysates Lysates/proteins at 20 μg per lane. (MITF Antikörper (N-Term))

MITF Antibody (N-term) (ABIN655506 and ABIN2845022) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). (MITF Antikörper (N-Term))

FACS

Confocal immunofluorescent analysis of MITF Antibody (N-term) (ABIN655506 and ABIN2845022) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (MITF Antikörper (N-Term))

MITF Antibody (N-term) (ABIN655506 and ABIN2845022) western blot analysis in K562 cell line lysates (35 μg/lane). (MITF Antikörper (N-Term))

Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1 A. (MITF Antikörper (N-Term))

IHC

5 Abbildungen

(1 reference)

Produktnummer ABIN655506

Datenblatt (PDF)

Target Alle MITF Antikörper anzeigen

MITF (Microphthalmia-Associated Transcription Factor (MITF))
Bindungsspezifität
15

15

13

9

7

5

4

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1
AA 1-28, N-Term
Reaktivität
99

49

24

17

5

5

5

5

4

4

4

3

2

2

2

2
Human
Wirt
75

39

1
Kaninchen
Passende Sekundärantikörper
Klonalität
66

49
Polyklonal
Konjugat
62

7

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
Dieser MITF Antikörper ist unkonjugiert
Applikation
69

47

41

35

35

15

13

7

7

3

3

2

2

2

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Homologie

M, Rat

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human MITF.

Klon

RB18810

Isotyp

Ig Fraction

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MITF Reaktivität: Human, Maus, Ratte, Affe WB, IF, IHC, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated

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MITF Reaktivität: Human WB, IF, IHC Wirt: Kaninchen Monoclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibody
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anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibody
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anti-Microphthalmia-Associated Transcription Factor (MITF) antibody
MITF Reaktivität: Human, Hund IF, IHC, FACS, StM Wirt: Kaninchen Monoclonal MITF-2987R unconjugated Recombinant Antibody

anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibody
MITF Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibody
MITF Reaktivität: Human, Maus WB, IHC, ELISA, IF/ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-520) antibody
MITF Reaktivität: Human WB, IP Wirt: Kaninchen Polyclonal unconjugated

anti-Microphthalmia-Associated Transcription Factor (MITF) antibody
MITF Reaktivität: Human, Hund IHC, StM Wirt: Maus Monoclonal MITF-915 unconjugated

Applikationshinweise

IF: 1:10~50. WB: 1:1000. WB: 1:2000. IHC: 1:800. FC: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Ishii, Ryu, Suzuki: "Lactoferrin inhibits melanogenesis by down-regulating MITF in melanoma cells and normal melanocytes." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 95, Issue 1, pp. 119-125, (2017) (PubMed).
Target

MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung

MITF (MITF Produkte)

Hintergrund

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molekulargewicht

58795

Gen-ID

4286

NCBI Accession

NP_000239, NP_001171896, NP_006713, NP_937801, NP_937802, NP_937820, NP_937821

UniProt

O75030

Pathways

Chromatin Binding