Dinitrophenol Antikörper

Produktnummer ABIN636352

Produktdetails anti-Dinitrophenol Antikörper

Target: Dinitrophenol (DNP)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Dinitrophenol Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Immunogen: DNP antibody was raised in goat using Dinitrophenol (DNP) conjugate as the immunogen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:2,000
Optimal conditions should be determined by the investigator.

Kommentare:

This antibody does not react with its immunogens carrier.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as serum.

Vorsichtsmaßnahmen: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Details zu Dinitrophenol

Target: Dinitrophenol (DNP)

Andere Bezeichnung: DNP (DNP Produkte)

Substanzklasse: Chemical

Hintergrund: Tissues are continually exposed to reactive oxygen species (ROS) that are produced in tissues from metabolism processes or the inflammatory response of leukocytes or macrophages. These ROS can cause damage to various cellular components. Dinitrophenylhydrazine (DNPH) will react with free carbonyl groups on proteins, leading to adduction of the dinitrophenyl (DNP) group to the carbonyl group. Using an antibody to DNP, it is possible to visualize and localize the sites of oxidative damage by immunodetection of DNP adduction resulting from reaction of the tissue with DNPH.