MCM10 Antikörper (Middle Region)

Produktnummer ABIN634085

Der Kaninchen Polyklonal anti-MCM10 Antikörper wird verwendet zum Nachweis von MCM10 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für MCM10 Antikörper (Middle Region) (ABIN634085)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MCM10 Antikörper

Bindungsspezifität: Middle Region

Spezifität: MCM10 antibody was raised against the middle region of MCM10

Aufreinigung: Affinity purified

Immunogen: MCM10 antibody was raised using the middle region of MCM10 corresponding to a region with amino acids PKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERV

Anwendungsinformationen

Applikationshinweise: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Kommentare:

MCM10 Blocking Peptide, (ABIN5614695), is also available for use as a blocking control in assays to test for specificity of this MCM10 antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of MCM10 antibody in PBS

Konzentration: Lot specific

Buffer: PBS

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Details zu MCM10

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Andere Bezeichnung: MCM10

Hintergrund: This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.

Molekulargewicht: 98 kDa (MW of target protein)

Pathways: Mitotic G1-G1/S Phases, DNA Replication