Actin Antikörper

Produktnummer ABIN6297628

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Actin in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Kaninchen.

Kurzübersicht für Actin Antikörper (ABIN6297628)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reaktivität: Human, Maus, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Actin Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Rabbit/Rat Muscle Actin Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.

Immunogen: SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-1 μg/mL
FACS:0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: Actin, alpha skeletal muscle

Hintergrund:

Target Description: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Gene Symbol: ACTA1///ACTA

Gen-ID: 58

UniProt: P68133

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development