Histone H1 Antikörper

Produktnummer ABIN6296764

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Histone H1 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Histone H1 Antikörper (ABIN6296764)

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Histone H1 Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Rat Histone Antibody [Sodium Azide Free]

Spezifität: Nuclear

Aufreinigung: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

Anwendungsinformationen

Applikationshinweise: Western blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Histone H1

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Andere Bezeichnung: Histone H1.0

Hintergrund:

Target Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Gene Symbol: H1F0///H1FV

Gen-ID: 3005

UniProt: P07305