Vimentin Antikörper

Produktnummer ABIN6296593

Dieser Maus Monoklonal Antikörper detektiert spezifisch Vimentin in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Schwein, Rind (Kuh), Huhn, Hund, Cat und Ziege.

Kurzübersicht für Vimentin Antikörper (ABIN6296593)

Target: Vimentin (VIM)

Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-Vimentin Antikörper

Verwendungszweck: Mouse anti-Human/Cow/Dog/Cat/Pig/Goat/Chicken VIM Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: Human recombinant protein was used as the immunogen for this VIM antibody.

Immunogen: Human recombinant protein was used as the immunogen for this VIM antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund:

Target Description: This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Gene Symbol: VIM

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose