NEFH Antikörper

Produktnummer ABIN6296241

Dieses Anti-NEFH-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NEFH in IHC und FACS. Geeignet für Human, Ratte, Maus, Huhn, Schwein, Rind (Kuh), Kaninchen, Meerschweinchen, Wüstenrennmaus und Cat.

Kurzübersicht für NEFH Antikörper (ABIN6296241)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Schwein, Rind (Kuh), Kaninchen, Meerschweinchen, Wüstenrennmaus, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-NEFH Antikörper

Verwendungszweck: Mouse anti-Human/Mouse/Rat/Guinea pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken NF-H Antibody (phospho) [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Immunogen: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament heavy polypeptide

Hintergrund:

Target Description: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Gene Symbol: NEFH///KIAA0845///NFH

Gen-ID: 4744

UniProt: P12036