MUC1 Antikörper

Produktnummer ABIN6296211

Der Maus Monoklonal anti-MUC1 Antikörper (ABIN6296211) detektiert spezifisch MUC1 in IHC, ELISA, FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für MUC1 Antikörper (ABIN6296211)

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-MUC1 Antikörper

Verwendungszweck: Mouse anti-Human/Mouse MUC-1 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic and cell surface

Aufreinigung: Breast cancer cell line ZR75-1 was used as the immunogen for the anti-MUC-1 antibody.

Immunogen: Breast cancer cell line ZR75-1 was used as the immunogen for the anti-MUC-1 antibody.

Anwendungsinformationen

Applikationshinweise: ELISA: use Ab at 2-5 μg/mL, order BSA-free format
Flow Cytometry: 0.25-0.5 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: Mucin-1 ]

Hintergrund:

Target Description: This mAb reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Gene Symbol: MUC1///PUM

Gen-ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling