CD34 Antikörper

Produktnummer ABIN6295964

Der Maus Monoklonal anti-CD34 Antikörper wird verwendet zum Nachweis von CD34 in Proben von Human, Cynomolgus und Rhesusaffen. Er wurde validiert für FACS, IHC und IF.

Kurzübersicht für CD34 Antikörper (ABIN6295964)

Target: CD34

Reaktivität: Human, Cynomolgus, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CD34 Antikörper

Verwendungszweck: Mouse anti-Human/Cynomolgus Monkey/Rhesus Monkey CD34 Antibody [Sodium Azide Free]

Spezifität: Cell surface

Aufreinigung: A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Immunogen: A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Anwendungsinformationen

Applikationshinweise: FACS: use antibody at 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu CD34

Target: CD34

Andere Bezeichnung: Hematopoietic progenitor cell antigen CD34

Hintergrund:

Target Description: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi 's sarcoma, but with a lower specificity.

Gene Symbol: CD34

Gen-ID: 947

UniProt: P28906