KRT17 Antikörper

Produktnummer ABIN6295932

Dieses Anti-KRT17-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KRT17 in WB, IHC, IF und FACS. Geeignet für Human, Ratte, Rind (Kuh), Schwein und Ziege.

Kurzübersicht für KRT17 Antikörper (ABIN6295932)

Target: KRT17 (Keratin 17 (KRT17))

Reaktivität: Human, Ratte, Rind (Kuh), Schwein, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT17 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-KRT17 Antikörper

Verwendungszweck: Mouse anti-Human/Rat/Cow/Goat/Pig Cytokeratin 17 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic

Aufreinigung: A cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.

Immunogen: A cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu KRT17

Target: KRT17 (Keratin 17 (KRT17))

Andere Bezeichnung: Keratin, type I cytoskeletal 17

Hintergrund:

Target Description: Cytokeratin 17 (CK17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IF's). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. CK17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered an epithelial stem cell marker because CK17 Ab marks basal cell differentiation. CK17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5&6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. CK17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20 % of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype.

Gene Symbol: KRT17

Gen-ID: 3872

UniProt: Q04695