Myogenin Antikörper (AA 138-158)

Produktnummer ABIN6294312

Dieses Anti-Myogenin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Myogenin in WB, ELISA, IHC, IF, FACS und IP. Geeignet für Human, Maus, Ratte, Schwein, Cat und Hund.

Kurzübersicht für Myogenin Antikörper (AA 138-158) (ABIN6294312)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte, Schwein, Cat, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myogenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Produktdetails anti-Myogenin Antikörper

Bindungsspezifität: AA 138-158

Verwendungszweck: Mouse anti-Human/Mouse/Rat/Dog/Cat/Pig Myogenin Antibody [Sodium Azide Free]

Spezifität: Nuclear

Aufreinigung: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.

Immunogen: Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.

Anwendungsinformationen

Applikationshinweise: ELISA: order BSA-free format
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: Myogenin

Hintergrund:

Target Description: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Gene Symbol: MYOG///BHLHC3///MYF4

Gen-ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development