WT1 Antikörper (AA 1-181)

Produktnummer ABIN6294230

Der Maus Monoklonal Anti-WT1-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, WT1 in Proben von Human zu detektieren.

Kurzübersicht für WT1 Antikörper (AA 1-181) (ABIN6294230)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-WT1 Antikörper

Bindungsspezifität: AA 1-181

Verwendungszweck: Mouse anti-Human Wilms Tumor 1 Antibody [Sodium Azide Free]

Spezifität: Cytoplasmic, membranous

Aufreinigung: A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.

Immunogen: A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: In 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: Wilms tumor protein

Hintergrund:

Target Description: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Gene Symbol: WT1

Gen-ID: 7490

UniProt: P19544

Pathways: Tube Formation