Combined Host Cell Proteins Antikörper (Biotin)

Produktnummer ABIN6266438

Hersteller Produkt- Nr.: 200-406-m61

Produktdetails

Target: Combined Host Cell Proteins

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB)

Hersteller Produkt- Nr.: 200-406-m61

Hersteller: Rockland

Verwendungszweck: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.

Produktmerkmale: Synonyms: rabbit anti-Host Cell Protein antibody Biotin conjugation, rabbit anti-HCP antibody, rabbit anti-E.Coli HCP antibody biotin conjugated, Anti-HCP biotin

Aufreinigung: Host Cell Protein A purified antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins.

Immunogen:

Immunogen: Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and has been tested by western blot and is suitable for ELISA.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:1000-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: Combined Host Cell Proteins

Hintergrund: Background: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.