TRBP Antikörper (Internal Region)

Produktnummer ABIN6265719

Der Kaninchen Polyklonal anti-TRBP Antikörper wird verwendet zum Nachweis von TRBP in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF und ICC.

Kurzübersicht für TRBP Antikörper (Internal Region) (ABIN6265719)

Target: TRBP (TARBP2P) (TAR (HIV-1) RNA Binding Protein 2 Pseudogene (TARBP2P))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-TRBP Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TRBP Antibody

Spezifität: TRBP Antibody detects endogenous levels of total TRBP.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRBP, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu TRBP

Target: TRBP (TARBP2P) (TAR (HIV-1) RNA Binding Protein 2 Pseudogene (TARBP2P))

Andere Bezeichnung: TRBP

Hintergrund:

Description: Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

Gene: TARBP2

Molekulargewicht: 43-46 kDa

Gen-ID: 6895

UniProt: Q15633

Pathways: Regulatorische RNA Pathways, Ribonucleoprotein Complex Subunit Organization