SIRT5 Antikörper (Internal Region)

Produktnummer ABIN6265094

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SIRT5 in WB, IHC, ELISA, ICC und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für SIRT5 Antikörper (Internal Region) (ABIN6265094)

Target: SIRT5 (Sirtuin 5 (SIRT5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-SIRT5 Antikörper

Bindungsspezifität: Internal Region

Spezifität: SIRT5 Antibody detects endogenous levels of total SIRT5.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SIRT5, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Andere Bezeichnung: SIRT5

Hintergrund:

Description: NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693, PubMed:29180469). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Activates SHMT2 by mediating its desuccinylation (PubMed:29180469). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity, however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

Gene: SIRT5

Molekulargewicht: 34 kDa

Gen-ID: 23408

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interaktom