RUNX2 Antikörper (Internal Region)

Produktnummer ABIN6264882

Der Kaninchen-Polyklonal Anti-RUNX2-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet zum Nachweis von RUNX2 in Proben aus Human, Maus und Ratte. }

Kurzübersicht für RUNX2 Antikörper (Internal Region) (ABIN6264882)

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RUNX2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RUNX2 Antikörper

Bindungsspezifität: Internal Region

Spezifität: RUNX2 Antibody detects endogenous levels of total RUNX2.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RUNX2, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Andere Bezeichnung: RUNX2

Hintergrund:

Description: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed:28505335, PubMed:28738062, PubMed:28703881). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

Gene: RUNX2

Molekulargewicht: 56 kDa

Gen-ID: 860

UniProt: Q13950