LC3A / LC3B (N-Term) Antikörper

Produktnummer ABIN6262925

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LC3A / LC3B in IF, WB, ICC und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für LC3A / LC3B (N-Term) Antikörper (ABIN6262925)

Target: LC3A / LC3B

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: N-Term

Verwendungszweck: LC3A/B Antibody

Spezifität: LC3A/B Antibody detects endogenous levels of total LC3A/B.

Homologie: Pig,Zebrafish,Bovine,Sheep,Dog,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LC3B, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Referenzen

Tang, Cui, Luan, Ruan, Wang, Yang, Wang, Liu, Wang: "Human tissue kallikrein 1 ameliorates erectile function via modulation of macroautophagy in aged transgenic rats." in: Andrology, (2018) (PubMed).

Feng, Lu, He, Wang, Qi, Pi, Zhang: "Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells." in: Frontiers in cellular and infection microbiology, Vol. 8, pp. 201, (2018) (PubMed).

Shao, Yang, Xu, Zhu, Huang, Li, Wu, Yue, Xiong, Chen, Kuang, Fan, Zhu, Zeng: "Inhibition of mTORC1 signaling protects kidney from irradiation-induced toxicity via accelerating recovery of renal stem-like cells." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 219, (2018) (PubMed).

Wu, Zheng, Bai, Xia, Zhang, Guo: "HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma." in: OncoTargets and therapy, Vol. 11, pp. 4501-4510, (2018) (PubMed).

Antigendetails

Target: LC3A / LC3B

Andere Bezeichnung: LC3A/B

Hintergrund:

Description: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene: MAP1LC3B

Molekulargewicht: 14.16 kDa

Gen-ID: 84557, 81631

UniProt: Q9H492