HMGB3 Antikörper (Internal Region)

Produktnummer ABIN6262302

Der Kaninchen-Polyklonal Anti-HMGB3-Antikörper wurde für WB, ELISA, ICC und IF validiert. Er ist geeignet zum Nachweis von HMGB3 in Proben aus Human und Maus. }

Kurzübersicht für HMGB3 Antikörper (Internal Region) (ABIN6262302)

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-HMGB3 Antikörper

Bindungsspezifität: Internal Region

Spezifität: HMGB3 Antibody detects endogenous levels of total HMGB3.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HMGB3, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Andere Bezeichnung: HMGB3

Hintergrund:

Description: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).

Gene: HMGB3

Molekulargewicht: 23 kDa

Gen-ID: 3149

UniProt: O15347