Histone H3.3 Antikörper (N-Term)

Produktnummer ABIN6262140

Der Kaninchen Polyklonal anti-Histone H3.3 Antikörper wird verwendet zum Nachweis von Histone H3.3 in Proben von Human und Maus. Er wurde validiert für WB, IHC, IF und ICC.

Kurzübersicht für Histone H3.3 Antikörper (N-Term) (ABIN6262140)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H3.3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Histone H3.3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: H3F3A Antibody

Spezifität: H3F3A Antibody detects endogenous levels of total H3F3A.

Homologie: Pig,Zebrafish,Bovine,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human H3F3A, corresponding to a region within N-terminal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IHC 1:50-1:200 IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Andere Bezeichnung: H3F3A

Hintergrund:

Description: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: H3F3A, H3F3B

Molekulargewicht: 19 kDa

Gen-ID: 3020, 3021

UniProt: P84243