CD34 Antikörper

Produktnummer ABIN626193

Dieser Anti-CD34 Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von CD34 in FACS, WB, IHC, IP, IHC (p) und IHC (fro). Geeignet für Human.

Kurzübersicht für CD34 Antikörper (ABIN626193)

Target: CD34

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: QBEnd-10

Produktdetails anti-CD34 Antikörper

Marke: IHC-plus™

Spezifität: The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on hematopoietic cells and on the established myeloid leukemic cell line KG1A. QBEND-10 recognizes the Class II epitope on CD34. This antibody does not cross-react with tested species such as sheep, cow, dog and rat. Clone is reported to cross react with Rhesus Monkey (2). Immunogen: human endothelial vesicles.

Aufreinigung: Purified

Immunogen: Human endothelial vesicles.

Type of Immunogen: Membrane preparation

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-Fr, IHC-P (1:800), IP, WB

Usage: The antibody can be used for flow cytometry, immunohistochemistry on frozen and paraffin embedded section, immunoprecipitation and Western blotting. Instructions for use Flow cytometry: Use 10 μL antibody (1:100-1:200 dilution) to label 106 cells in 100 μL Immunohistochemistry: This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended dilution: 1:500 - 1:1000 for immunohistochemistry on paraffin sections. Recommended positive control tissue: Tonsil, bone marrow.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: Name/Gene ID: CD34

Synonyms: CD34, CD34 Molecule, CD34 antigen

Gen-ID: 947

UniProt: P28906