SR Proteins Antikörper

Produktnummer ABIN626151

Der Maus Monoklonal anti-SR Proteins Antikörper (ABIN626151) detektiert spezifisch SR Proteins in IF, ELISA, WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Rind (Kuh), Maus, Kaninchen und Ratte.

Kurzübersicht für SR Proteins Antikörper (ABIN626151)

Target: SR Proteins

Reaktivität: Human, Rind (Kuh), Maus, Kaninchen, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Spezifität: Detects a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species cross-reactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.

Aufreinigung: Protein A purified

Immunogen: Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST.

Type of Immunogen: GST fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF (0.5 - 2 μg/mL), IHC, IHC-P (10 μg/mL), WB (3 - 5 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot. Reactivity is confirmed by Western Blot. ELISA: 0.1-1 μg/mL. Immunofluorescence: 0.5-2 μg/mL, human. Western Blot: 3-5 μg/mL. Positive control: HeLa cell, nuclear extracts.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Antigendetails

Target: SR Proteins