CD3G Antikörper (Internal Region)

Produktnummer ABIN6260620

Dieses Anti-CD3G-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CD3G in WB, ELISA, IHC, ICC und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CD3G Antikörper (Internal Region) (ABIN6260620)

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD3G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-CD3G Antikörper

Bindungsspezifität: Internal Region

Spezifität: CD3G Antibody detects endogenous levels of total CD3G.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CD3G, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu CD3G

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Andere Bezeichnung: CD3G

Hintergrund:

Description: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

Gene: CD3G

Molekulargewicht: 20 kDa

Gen-ID: 917

UniProt: P09693

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events