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MHC Class II RT-1B Antikörper

Der Maus Monoklonal Anti-MHC Class II RT-1B-Antikörper wurde für FACS, IHC (fro), IHC (p), WB und IHC validiert. Er ist geeignet, MHC Class II RT-1B in Proben von Ratte und Maus zu detektieren.
Produktnummer ABIN625901

Kurzübersicht für MHC Class II RT-1B Antikörper (ABIN625901)

Target

MHC Class II RT-1B

Reaktivität

Ratte, Maus

Wirt

  • 9
Maus

Klonalität

  • 9
Monoklonal

Konjugat

  • 3
  • 3
  • 2
  • 1
Dieser MHC Class II RT-1B Antikörper ist unkonjugiert

Applikation

  • 9
  • 4
  • 3
  • 3
  • 2
  • 2
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Klon

OX-6
  • Marke

    IHC-plus™

    Spezifität

    Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.

    Aufreinigung

    Protein G purified

    Immunogen

    Rat thymocyte membrane glycoprotein.

    Type of Immunogen: Membrane extract

    Isotyp

    IgG1
  • Applikationshinweise

    Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

    Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer  pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)

    Kommentare

    Target Species of Antibody: Rat

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 0.09 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeat freeze-thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    MHC Class II RT-1B
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