OCT4 Antikörper (AA 114-163)

Produktnummer ABIN625854

Dieses Anti-OCT4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von OCT4 in WB, IHC und IHC (p). Geeignet für Human, Schwein, Kaninchen und Ziege.

Kurzübersicht für OCT4 Antikörper (AA 114-163) (ABIN625854)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human, Schwein, Kaninchen, Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-OCT4 Antikörper

Bindungsspezifität: AA 114-163

Marke: IHC-plus™

Spezifität: Human POU5F1 / OCT4

Homologie: Percent identity by BLAST analysis: Human, Goat (100%) Rabbit (92%) Dog (91%) Pig (84%).

Aufreinigung: Protein A/G purified

Immunogen: Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%), Marmoset (92%), Dog (91%), Bovine, Pig, Guinea pig (85%), Horse (84%), Cat (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: PBS, 2 % sucrose.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: POU5F1 / OCT4

Hintergrund: Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

Gen-ID: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance