SDF4 Antikörper (AA 161-175)

Produktnummer ABIN625824

Dieses Anti-SDF4-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von SDF4 in WB, ELISA, IHC, FACS und IF. Geeignet für Human.

Kurzübersicht für SDF4 Antikörper (AA 161-175) (ABIN625824)

Target: SDF4 (Stromal Cell Derived Factor 4 (SDF4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SDF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-SDF4 Antikörper

Bindungsspezifität: AA 161-175

Verwendungszweck: SDF4 (aa161-175)

Sequenz: EYKVKFLASK GHSEK

Spezifität: This antibody is expected to recognize both reported isoforms NP_057631.2, and NP_057260.3

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: CEYKVKFLASKGHSEK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8 kDa according to NP_057260.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SDF4

Target: SDF4 (Stromal Cell Derived Factor 4 (SDF4))

Andere Bezeichnung: SDF4

Hintergrund: SDF4, stromal cell derived factor 4, Cab45, 45 kDa calcium-binding protein, OTTHUMP00000001754, SDF-4, calcium binding protein, stromal cell-derived factor 4

Gen-ID: 51150, 20318, 155173

NCBI Accession: NP_057631, NP_057260