CDC42BPA Antikörper (Internal Region)

Produktnummer ABIN6258012

Der Kaninchen Polyklonal anti-CDC42BPA Antikörper wird verwendet zum Nachweis von CDC42BPA in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC, ICC und IF.

Kurzübersicht für CDC42BPA Antikörper (Internal Region) (ABIN6258012)

Target: CDC42BPA (CDC42 Binding Protein Kinase alpha (DMPK-Like) (CDC42BPA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC42BPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-CDC42BPA Antikörper

Bindungsspezifität: Internal Region

Spezifität: CDC42BPA Antibody detects endogenous levels of total CDC42BPA.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CDC42BPA, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu CDC42BPA

Target: CDC42BPA (CDC42 Binding Protein Kinase alpha (DMPK-Like) (CDC42BPA))

Andere Bezeichnung: CDC42BPA

Hintergrund:

Description: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).

Gene: CDC42BPA

Molekulargewicht: 97 kDa

Gen-ID: 8476

UniProt: Q5VT25