Crossover junction endonuclease EME1 (EME1) (Internal Region) Antikörper

Produktnummer ABIN6256869

Der Kaninchen Polyklonal Anti--Antikörper wurde für WB, ELISA, IF und ICC validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Crossover junction endonuclease EME1 (EME1) (Internal Region) Antikörper (ABIN6256869)

Target: Crossover junction endonuclease EME1 (EME1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails

Bindungsspezifität: Internal Region

Spezifität: EME1 Antibody detects endogenous levels of total EME1.

Homologie: Rabbit,Dog

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EME1, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Crossover junction endonuclease EME1 (EME1)

Andere Bezeichnung: EME1

Hintergrund:

Description: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Gene: EME1

Molekulargewicht: 65 kDa

Gen-ID: 146956

UniProt: Q96AY2