NOS2 Antikörper (pTyr151)

Produktnummer ABIN6256645

Dieses Anti-NOS2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NOS2 in WB, IHC und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für NOS2 Antikörper (pTyr151) (ABIN6256645)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-NOS2 Antikörper

Bindungsspezifität: pTyr151

Spezifität: Phospho-iNOS (Tyr151) Antibody detects endogenous levels of iNOS only when phosphorylated at Tyrosine 151.

Homologie: Bovine,Horse,Sheep,Dog

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human iNOS around the phosphorylation site of Tyr151.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Andere Bezeichnung: NOS2

Hintergrund:

Description: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).

Gene: NOS2

Molekulargewicht: kDa

Gen-ID: 4843

UniProt: P35228

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process