WNK1 Antikörper (pThr60)

Produktnummer ABIN6256585

Dieses Anti-WNK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von WNK1 in WB, ELISA, IHC, IF und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für WNK1 Antikörper (pThr60) (ABIN6256585)

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-WNK1 Antikörper

Bindungsspezifität: pThr60

Spezifität: Phospho-WNK1 (Thr60) Antibody detects endogenous levels of WNK1 only when phosphorylated at Threonine 60.

Homologie: Pig,Bovine,Sheep,Rabbit,Dog,Chicken

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human WNK1 around the phosphorylation site of Thr60.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Andere Bezeichnung: WNK1

Hintergrund:

Description: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity).

Gene: WNK1

Molekulargewicht: 230kDa

Gen-ID: 65125

UniProt: Q9H4A3