CAMK4 Antikörper (pThr200)

Produktnummer ABIN6256500

Der Kaninchen Polyklonal Anti-CAMK4-Antikörper wurde für WB, ELISA, IHC, IF und ICC validiert. Er ist geeignet, CAMK4 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CAMK4 Antikörper (pThr200) (ABIN6256500)

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAMK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-CAMK4 Antikörper

Bindungsspezifität: pThr200

Spezifität: Phospho-CaMK4 (Thr200) Antibody detects endogenous levels of CaMK4 only when phosphorylated at Threonine 200.

Homologie: Pig,Zebrafish,Horse,Sheep,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CaMK4 around the phosphorylation site of Thr200.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu CAMK4

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Andere Bezeichnung: CAMK4

Hintergrund:

Description: Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

Gene: CAMK4

Molekulargewicht: 60kDa

Gen-ID: 814

UniProt: Q16566

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma