Retinoblastoma Protein (Rb) Antikörper (pSer807)

Produktnummer ABIN6256428

Der Kaninchen Polyklonal anti-Retinoblastoma Protein (Rb) Antikörper wird verwendet zum Nachweis von Retinoblastoma Protein (Rb) in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF und ICC.

Kurzübersicht für Retinoblastoma Protein (Rb) Antikörper (pSer807) (ABIN6256428)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Retinoblastoma Protein (Rb) Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Retinoblastoma Protein (Rb) Antikörper

Bindungsspezifität: pSer807

Verwendungszweck: Phospho-Retinoblastoma (Ser807) Antibody

Spezifität: Phospho-Retinoblastoma (Ser807) Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Serine 807.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser807.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Andere Bezeichnung: Retinoblastoma

Hintergrund:

Description: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Gene: RB1

Molekulargewicht: 106kDa

Gen-ID: 5925

UniProt: P06400

Pathways: Mitotic G1-G1/S Phases