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IKK-alpha /IKK-beta Antikörper (pSer180, pSer181)

Dieses Anti-IKK-alpha /IKK-beta-Antikörper ist ein Rabbit Polyclonal-Antikörper zur Detektion von IKK-alpha /IKK-beta in WB, IHC, ELISA, ICC, IF. Geeignet für Mouse, Human, Rat.
Produktnummer ABIN6255040

Kurzübersicht für IKK-alpha /IKK-beta Antikörper (pSer180, pSer181) (ABIN6255040)

Target

IKK-alpha /IKK-beta

Reaktivität

  • 73
  • 47
  • 27
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Maus, Human, Ratte

Wirt

  • 78
Kaninchen

Klonalität

  • 69
  • 9
Polyklonal

Konjugat

  • 22
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser IKK-alpha /IKK-beta Antikörper ist unkonjugiert

Applikation

  • 49
  • 41
  • 40
  • 21
  • 16
  • 11
  • 10
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
  • Bindungsspezifität

    • 34
    • 25
    • 17
    • 15
    • 10
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer180, pSer181

    Spezifität

    Phospho-IKK- alpha/ beta (Ser180/181) Antibody detects endogenous levels of IKK- alpha/ beta only when phosphorylated at Serine 180/181

    Kreuzreaktivität

    Human, Maus, Ratte (Rattus)

    Aufreinigung

    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

    Immunogen

    A synthesized peptide derived from human IKK- alpha/ beta around the phosphorylation site of Serine 180/181

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store at -20 °C.Stable for 12 months from date of receipt

    Haltbarkeit

    12 months
  • Target

    IKK-alpha /IKK-beta

    Andere Bezeichnung

    IKK alpha/ beta

    Hintergrund

    Description: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor (PubMed:15084260).

    Gene: CHUK

    Molekulargewicht

    85kDa

    Gen-ID

    1147

    UniProt

    O15111, O14920
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